Translational_Unit
gpS

Part:BBa_K836006:Design

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)

Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 275
    Illegal BsaI.rc site found at 188


Design Notes

Between the RBS and the CDS a spacer consisting on a scar from a standard suffix and a prefix for CDS was inserted.


Source

The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/P03705#section_seq lysis protein S) and optimized for R. opacus (avoiding standard restriction sites) using the free software GeneDesigner from DNA 2.0.

References